Aquagenx CBT EC+TC Kit

Easier, Faster E. coli Test Results in the Field

Compare the Aquagenx CBT EC+TC Kit to Other Tests

 

The CBT EC+TC Kit is simple and easy to use to detect E. coli and total coliform bacteria in the field.

It does not need electricity, a lab, cold chain or expensive, complicated equipment or intensive training.

See how our CBT EC+TC Kits compare to other P/A and MPN tests for E. coli and total coliforms.

Compare How-To Videos of 100 mL Tests for E. coli and Total Coliforms

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Aquagenx® CBT
EC+TC Kit

Wells in Trays
or Plates

Membrane Filtration Methods  Multiple Tube
Testing method Presence/Absence (P/A) and Most Probable Number (MPN) Most Probable Number (MPN) with Presence/Absence (P/A) versions Colony Forming Units (CFU) Most Probable Number (MPN)
Microbial indicator E. coli and total coliforms E. coli and total coliforms Depends on product/system: Thermotolerant (fecal) Coliforms; E. coli Depends on the differential and selective culture media used. Available for E. coli, thermotolerant (fecal) coliforms, coliforms and other fecal bacteria
Usability Easy for anyone to use, designed for field testing. No color comparators are required, color-change test results easy to identify. Requires intensive training. Color comparator needed for MPN and P/A test results. Requires intensive training, challenging to use for field testing Requires intensive training and is impractical or impossible for field use. Extra materials are needed (see components below) sample bottles, culture tubes and racks, pipets, diluent for serial dilutions, controlled temperature incubation and in some tests, further confirmatory testing.
Portability Lightweight, easy to carry and transport Lab use only Can be heavy and/or cumbersome, multiple components Lab use only
Incubation Ambient temperature incubation >25° Celsius Requires incubator and temperature control Requires incubator and temperature control Requires incubator and temperature control
Components Aquagenx EC+TC medium, Thio-Bag, Aquagenx Compartment Bag for MPN testing, Seal Clip for MPN testing, UV flashlight (365nm) for total coliform detection Requires multi-well trays, tray comparator, rubber insert, and special plate sealer for MPN results, 6-watt UV lamp for E. coli detection in some samples, color comparator for P/A results, rigid sample containers Filter and funnel apparatus, vacuum system (vacuum flask and vacuum source, typically an electrical pump or a vacuum line in a lab) or large syringe, culture plates, petri dishes, gridded membrane filters, multiple measuring devices, absorbent pads, volume dispensers (e.g., sterile pipettes and/or graduated cylinders or centrifuge tubes with volume marks), tweezers, heat source to flame tweezers Sample bottles, multiple culture tubes and racks, pipets, diluent for serial dilutions, controlled temperature incubator and in some tests, further confirmatory testing
Procedures Easy, small number of steps. No color comparators required
Determine E. coli results in ambient light
Determine total coliform results that are negative for E. coli with UV flashlight
P/A testing: use color comparator to obtain  total coliform results, use UV light to obtain E. coli results
MPN testing: tray and rubber insert preparation, sealer procedure, controlled incubation procedure, controlled incubation conditions
Time consuming with multiple steps: equipment set-up and preparation, culture media preparation, sterilization of multiple pieces of equipment Time consuming with multiple steps: Making, autoclaving or boiling culture media, dispensing culture media into tubes set up in racks, adding samples and their dilutions to culture media tubes, and incubating at controlled temperatures. Some tests require further transfer of positive culture tubes to additional culture tubes for confirmation.
Growth medium storage Store at 10-25° Celsius in a dry environment, refrigeration is optional Store at 2-30° Celsius Requires refrigeration and cold chain Culture media can be stored for weeks at refrigerated temperatures, but dehydration and extraneous contamination is possible
Post-test disposables and reusables No reusables to clean or sterilize. To decontaminate sample, add liquid bleach or chlorine tablets to Thio-Bag or Compartment Bag. Or, treat with heat >60°C. After 30 minutes, pour contents into a sink, toilet or hole in ground and safely dispose the bags. Trays should be decontaminated prior to disposal, typically by autoclaving Disposables: Petri dishes (and possibly sample collection bag, large syringe for pulling a vacuum, disposable pipettes and disposable graduated centrifuge tubes) that must be decontaminated prior to disposal (e.g., with heat >60 degrees C or free chlorine). Reusables: Multiple materials and equipment that require decontamination, cleaning and sterilization (e.g., filter apparatus, pipettes and/or graduated cylinders, vacuum flask, sample collection bottles, etc.) Disposables: culture tubes, pipets, sample bottles, and in some tests, inoculating loops that must be decontaminated prior to disposable (e.g., with heat >60 degrees C or free chlorine). Reusables: multiple materials and equipment that require decontamination, cleaning and sterilization (culture tubes, sample bottles, dilution tubes, pipets, inoculating loops that must be sterile or flame-sterilized, and equipment maintenance and calibration (e.g., incubators).

CBT EC+TC Kit Components

Growth Medium

Aquagenx powder growth medium dissolves quickly

Compartment Bag

Aquagenx Compartment Bag included with MPN Kits

Whirl-Pak Thio-Bag

100 mL Whirl-Pak Thio-Bag for sample collection and P/A Kits

Seal Clip

Seal clip snaps on Compartment Bag to prevent leakage